Journal: Frontiers in Endocrinology

Article Title: Unveiling the TrkA-p35/CDK5 axis: a novel therapeutic target in diabetic kidney disease

doi: 10.3389/fendo.2026.1791283

Figure Lengend Snippet: HG-induced TrkA phosphorylation is associated with podocyte inflammation and injury. (A–D) Representative western blots show the levels of (A) TrkA and phospho-TrkA (Tyr490); (B) ERK, phospho-ERK and EGR1; (C) p35 and CDK5; and (D) IL-1β and TNF-α in MPC5 cells from the LG, HG, and LG + Man groups at 24 h (E) Representative immunofluorescence double staining shows the expression of phospho-TrkA (Tyr490) and synaptopodin in MPC5 cells from the LG, HG, and LG + Man groups. ****p < 0.0001. p < 0.05 was considered statistically significant; ns indicates not significant.

Article Snippet: Anti-phospho-TrkA (pTyr490) antibody (Cat. No. RA18018-100) was purchased from Origene (Rockville, MD, USA).

Techniques: Phospho-proteomics, Western Blot, Immunofluorescence, Double Staining, Expressing

Journal: Frontiers in Endocrinology

Article Title: Unveiling the TrkA-p35/CDK5 axis: a novel therapeutic target in diabetic kidney disease

doi: 10.3389/fendo.2026.1791283

Figure Lengend Snippet: TrkA inhibition reduces the levels of p-TrkA, p-ERK, EGR1, p35, and CDK5 in the kidneys of db/db mice. (A–C) Representative western blots show the protein expression levels of (A) TrkA and phospho-TrkA (Tyr490), (B) ERK, p-ERK and EGR1, and (C) p35 and CDK5 in the kidney samples from the control, db/db, Inh-NC, and Inh-TrkA groups of mice. (D) Representative IF images show the expression of Synaptopodin (red) in the glomeruli of control, db/db, Inh-NC, and Inh-TrkA groups of mice (400X). ***p < 0.001. p < 0.05 was considered statistically significant; ns indicates not significant.

Article Snippet: Anti-phospho-TrkA (pTyr490) antibody (Cat. No. RA18018-100) was purchased from Origene (Rockville, MD, USA).

Techniques: Inhibition, Western Blot, Expressing, Control

Journal: bioRxiv

Article Title: Multiple glycoforms of TrkA interact with N-cadherin during trigeminal ganglion neurodevelopment

doi: 10.1101/2025.11.14.688512

Figure Lengend Snippet: (A-C’) Representative transverse sections through the trigeminal ganglion of an E5.5/HH28 chick embryo following immunohistochemistry for N-cadherin (N-cad) and TrkA. Scale bar in (A) is 50 µm and applies to (B, C). Scale bar in (A’) is 50 µm and applies to (B’, C’). (D-I) Representative trigeminal ganglia explant cultures after immunocytochemistry for N-cadherin and TrkA. Scale bar in (D) is 25 µm and applies to (E-I). Arrowheads indicate colocalization of N-cadherin and TrkA. (J, K) Immunoblots of HH28-30 trigeminal ganglia for TrkA with lysate from the embryo ( in vivo ) (J) and from trigeminal ganglia explant cultures ( ex vivo ) (K). Pie charts represent percentage of total signal coming from the different bands of TrkA.

Article Snippet: The membrane was blocked in 5% milk (in 1X PBS+0.1% Tween-20 (PTW)) for 30 minutes at room temperature followed by overnight incubation at 4°C with the following primary antibodies diluted in blocking solution: N-cadherin (MNCD2, DSHB 1:100) and TrkA (MSU, 1:5000 or Origene, 1:1000).

Techniques: Immunohistochemistry, Immunocytochemistry, Western Blot, In Vivo, Ex Vivo

Journal: bioRxiv

Article Title: Multiple glycoforms of TrkA interact with N-cadherin during trigeminal ganglion neurodevelopment

doi: 10.1101/2025.11.14.688512

Figure Lengend Snippet: (A) Dissected trigeminal ganglion from an E5.5/HH28 chick embryo. Dotted lines indicate approximate location where cuts were made to separate trigeminal cell bodies from axons. Scale bar is 1mm. (B-C) N-cadherin-TrkA co-immunoprecipitation on pooled HH28-30 trigeminal cell bodies and axons. Input lanes contain 10% of lysate loaded in immunoprecipitation lanes. OpV, ophthalmic; MmV, maxillomandibular; IP, immunoprecipitation; IgG, Immunoglobulin G. N=3.

Article Snippet: The membrane was blocked in 5% milk (in 1X PBS+0.1% Tween-20 (PTW)) for 30 minutes at room temperature followed by overnight incubation at 4°C with the following primary antibodies diluted in blocking solution: N-cadherin (MNCD2, DSHB 1:100) and TrkA (MSU, 1:5000 or Origene, 1:1000).

Techniques: Immunoprecipitation

Journal: bioRxiv

Article Title: Multiple glycoforms of TrkA interact with N-cadherin during trigeminal ganglion neurodevelopment

doi: 10.1101/2025.11.14.688512

Figure Lengend Snippet: (A) Immunoblots of E6.5/HH28-30 trigeminal ganglia lysate following Mem-PER membrane extraction protocol for TrkA, N-cadherin, Tubb3, GM130 (Golgi protein), and SERCA2 (ER protein) using membrane-extracted and cytosolic-extracted protein fractions. (B) N-cadherin-TrkA co-immunoprecipitation on membrane-extracted fraction. Input contains 10% of lysate loaded in immunoprecipitation lanes. The top blot image is from one experiment using a high exposure and the bottom blot image is from a separate experiment with a lower exposure. IP, immunoprecipitation, IgG, Immunoglobulin G (N=3).

Article Snippet: The membrane was blocked in 5% milk (in 1X PBS+0.1% Tween-20 (PTW)) for 30 minutes at room temperature followed by overnight incubation at 4°C with the following primary antibodies diluted in blocking solution: N-cadherin (MNCD2, DSHB 1:100) and TrkA (MSU, 1:5000 or Origene, 1:1000).

Techniques: Western Blot, Membrane, Extraction, Immunoprecipitation

Journal: Pathogens

Article Title: Cellular Receptor Tyrosine Kinase Signaling Plays Important Roles in SARS-CoV-2 Infection

doi: 10.3390/pathogens14040333

Figure Lengend Snippet: Western blot analysis of RTK activation in A549-ACE2 cells after SARS-CoV-2 infection. Cell lysates from mock infection (Mock), A/PR8 (PR8), and SARS-CoV-2 infection at different time points were analyzed by Western blotting to detect active and total forms of EGFR ( A ), TrkA ( B ), and HER2 ( C ). Protein bands were quantified using Sciugo software 2.0.1. The ratio of the phosphorylated form to total form was calculated and compared to the mock (set as 1.00).

Article Snippet: The following antibodies were used for immunoblotting: rabbit anti-human TrkA polyclonal antibody (Cell Signaling Technology, 2505S, Danvers, MA, USA), rabbit anti-human pTrkA monoclonal antibody (mAb) (Cell Signaling Tech, 4168S), rabbit anti-human HER2 mAb (Cell Signaling Tech, 2165S), rabbit anti-human pHER2 mAb (Cell Signaling Tech, 2243L), mouse anti-human EGFR mAb (Cell Signaling Tech, 2239S), rabbit anti-human pEGFR mAb (Cell Signaling Tech, 3777S), rabbit anti-human GAPDH mAb (Cell Signaling Tech, 2118S), anti-rabbit HRP conjugated (Bio Rad, 1706515, Hercules, CA, USA), and anti-mouse IgG HRP conjugated (R&D, HAF007).

Techniques: Western Blot, Activation Assay, Infection, Software

Journal: International Journal of Molecular Sciences

Article Title: Molecular Characterization and Inhibition of a Novel Stress-Induced Mitochondrial Protecting Role for Misfolded TrkAIII in Human SH-SY5Y Neuroblastoma Cells

doi: 10.3390/ijms25105475

Figure Lengend Snippet: ( a ) Indirect IFs demonstrating increased overlapping (yellow/orange) immunoreactivity for TrkA (green) and MitoTracker-labeled mitochondria (red) (upper panels) and Y490 phosphorylated TrkAIII (pTrkAIII, green) and MitoTracker-labeled mitochondria (red) (lower panels) in DTT-treated (5 mM for 6 h) TrkAIII SH-SY5Y cells compared to untreated controls (control). DAPI stained nuclei are blue. (bar = 50 μm). ( b ) Western blots demonstrating TrkAIII cleavage and Y674/5 phosphorylation in mitochondria (50 mg) from DTT-treated (5 mM for 6 h) TrkAIII SH-SY5Y cells compared to untreated TrkAIII SH-SY5Y controls. ( c ) Western blots demonstrating increased PTPase oxidation (arrows) in mitochondria (50 mg) from DTT-treated TrkAIII SH-SY5Y cells (5 mM for 6 h) compared to untreated controls (Con). ( d ) Phase contrast images merged with green fluorescence and histogram demonstrating significant differences (* p < 0.0001) in pcDNA-SH-SY5Y and TrkAIII SH-SY5Y percentage cell death at 24 h and 48 h, induced by 5 mM DTT.

Article Snippet: Polyclonal rabbit anti-human Y490 phosphorylated TrkA (9141), polyclonal rabbit anti-human Akt (9272), polyclonal rabbit anti-human phospho-Ser 473-Akt (4060), polyclonal rabbit anti-human Y674/675 (4621), antibody was from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Labeling, Control, Staining, Western Blot, Phospho-proteomics, Fluorescence

Journal: International Journal of Molecular Sciences

Article Title: Molecular Characterization and Inhibition of a Novel Stress-Induced Mitochondrial Protecting Role for Misfolded TrkAIII in Human SH-SY5Y Neuroblastoma Cells

doi: 10.3390/ijms25105475

Figure Lengend Snippet: ( a ) Western blots demonstrating TrkAIII cleavage and phosphorylation in mitochondria (50 μg) from TrkAIII SH-SY5Y cells treated with 5 mM DTT alone for 6 h and in mitochondria from TrkAIII SH-SY5Y cells co-treated with 5 mM DTT and either HA-15 (20 μM) (DTT/HA); brefeldin A (5 mg/mL) (DTT/BfA) or W7 (60 μM) (DTT/W7) plus non-phosphorylated TrkAIII in mitochondria from untreated TrkAIII SH-SY5Y cells (Con) and lack of TrkA or phosphorylated TrkA immunoreactivity in mitochondria (50 μg) from untreated (Con) and DTT-treated (5 mm for 6 h) pcDNA-SH-SY5Y cells. ( b ) Line graphs demonstrating significant inhibition (*) of pcDNA-SH-SY5Y and TrkAIII SH-SY5Y proliferation by BfA (5 μg/mL) and W7 (60 μM) but not by HA-15 (20 μM) at 24 and 48 h (* p < 0.0001). ( c ) Phase contrast images merged with green fluorescence plus histograms demonstrating percentage pcDNA-SH-SY5Y and TrkAIII SH-SY5Y cell death induced by 5 mM DTT alone (DTT), DTT and either HA-15 (20 μM) (DTT/HA), BfA (5 μg/mL) (DTT/BfA) or W7 (60 μM) (DTT/W7), plus pcDNA-SH-SY5Y and TrkAIII SH-SY5Y cell death induced by W7 (60 μM) alone (W7) at 24 and 48 h (* p < 0.006).

Article Snippet: Polyclonal rabbit anti-human Y490 phosphorylated TrkA (9141), polyclonal rabbit anti-human Akt (9272), polyclonal rabbit anti-human phospho-Ser 473-Akt (4060), polyclonal rabbit anti-human Y674/675 (4621), antibody was from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Western Blot, Phospho-proteomics, Inhibition, Fluorescence

Journal: Inflammation and Regeneration

Article Title: Novel artificial nerve transplantation of human iPSC-derived neurite bundles enhanced nerve regeneration after peripheral nerve injury

doi: 10.1186/s41232-024-00319-4

Figure Lengend Snippet: Generation and implantation procedure of novel artificial nerves with motor and sensory neurite bundles. a Schematic diagram of creating nerve organoids with neurite bundles derived from spheroids. b Whole image of the nerve organoid cultured in the microfluidic device. Scale bar = 300 μm. c , Schematic explanation of the procedure for neurite bundle-derived artificial nerve creation by removing spheroids that contained cell bodies from the nerve organoids. d The implantation procedure of a nerve conduit tube at the transected sciatic nerve with a 13 mm gap. e Immunofluorescence images of ChAT and Islet1/2 staining of motor neuron spheroids derived from hiPSCs. Scale bars = 100 μm. f Quantitative analysis of ChAT- and Islet1/2-positive cells in pan-ELAVL-positive cells. g Immunofluorescence images of TrkA, TrkB, TrkC, PV (parvalbumin) and CGRP staining of sensory neuron spheroids derived from hiPSCs. Scale bars = 100 μm. h Quantitative results of TrkA-, TrkB-, TrkC-, PV (parvalbumin)- and CGRP-positive cells in pan-ELAVL-positive cells. i , Electron microscopic images of the axial section from motor and sensory neurite bundles consisting of condensed neural fibers. Scale bars = 1 μm. j Neurite diameter distribution in motor and sensory neurite bundles. Arrowheads indicate the position of the average diameter. k Average neurite density in neurite bundles. l , m Average ratio of neurofilaments’ area ( l ) and microtubules’ area ( m ) in neurite area. ( n = 5) * p < 0.05, N.S. = not significant. Data are represented as the mean ± standard error of the mean (SEM)

Article Snippet: The primary antibodies used in this study were human polyclonal anti-pan-ELAVL (ELAV-like protein 2/3/4) antibody (1:2000, kindly provided from Prof. Robert Darnell, Rockefeller University), rabbit polyclonal anti-TrkA antibody (1:500, kindly provided from Prof. Louis F. Reichardt, UCSF), chicken polyclonal anti-TrkB antibody (1:500, kindly provided from Prof. Louis F. Reichardt, UCSF), goat polyclonal anti-TrkC antibody (AF373, 1:200, R&D systems), rabbit polyclonal anti-parvalbumin antibody (PV-28, 1:1000, Swant), rabbit polyclonal anti-CGRP antibody (BML-CA1134, 1:500, Enzo Life Sciences), Goat polyclonal anti-Choline acetyltransferase antibody (AB144P, 1:200, Chemicon), Mouse monoclonal anti-Islet1 and Islet2 antibody (39.4D5, 1:200, DSHB), rabbit polyclonal anti-Neurofilament heavy polypeptide antibody (ab8135, 1:500, Abcam), goat polyclonal anti-mouse and anti-rat CD31 (AF3628, 1:100, R&D), rabbit polyclonal anti-Iba1 antibody (GtX100042, 1:500, GeneTex), goat anti-GFP antibody (600–101-215, 1:1000, Rockland).

Techniques: Derivative Assay, Cell Culture, Immunofluorescence, Staining